ABCB4 Antibody (Center)

SKU: orb1930247

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:500, IHC-P - 1:50-100
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD24421
• Immunogen: This ABCB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 624-654 amino acids from the Central region of human ABCB4. Antigen Region: 624-654 aa.
• Target: ABCB4 (HGNC:45)
• Molecular Weight: 141523 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Multidrug resistance protein 3, ATP-binding cassette sub-family B member 4, P-glycoprotein 3, ABCB4, MDR3, PGY3

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

ABCB4-S639 western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the ABCB4 antibody detected ABCB4 protein (arrow).

Anti-hABCB4-S639 at 1:2000 dilution + Human brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 142 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-hABCB4-S639 at 1:500 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 142 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ABCB4 (HGNC:45)

UniProt

P21439

RefSeq (Protein):NP_000434.1, NP_061337.1, NP_061338.1