ABCB10 Antibody (N-term)

• Catalog Number: orb1930250
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01807
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000-1:2000, IHC-P: 1:25, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 79148 Da
• Target: This ABCB10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human ABCB10.
• UniProt ID: Q9NRK6
• NCBI: NP_036221.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ATP-binding cassette sub-family B member 10, mitoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining hABCB10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

ABCB10 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ABCB10 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ABCB10 Antibody (S16) western blot analysis in MCF-7 cell line lysates (35 ug/lane). This demonstrates the ABCB10 antibody detected the ABCB10 protein (arrow).

All lanes: Anti-ABCB10 Antibody (S16) at 1:1000-1:2000 dilution. Lane 1: K562 whole cell lysates. Lane 2: Raji whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 79 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.