AAT rabbit pAb

• Catalog Number: orb766661
• Category: Antibodies
• Description: Rabbit polyclonal antibody to alpha 1 Antitrypsin
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Synthesized peptide derived from AAT . at AA range: 240-320
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 46
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-SERPINA1 antibody, anti-AAT antibody, anti-PI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Immunohistochemical analysis of paraffin-embedded Human Brain. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).