A2M Antibody / Alpha-2-Macroglobulin

• Catalog Number: orb699606
• Category: Antibodies
• Description: Alpha-2-Macroglobulin (A2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, it was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on A2M. This interaction induces a conformational change, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, A2M has been shown to function as a carrier protein and regulator of cytokines during inflammation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2c, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 604-748 from the human protein was used as the immunogen for the Alpha-2-Macroglobulin antibody.
• UniProt ID: P01023
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the Alpha-2-Macroglobulin antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: A2M/3621
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC staining of FFPE human liver with Alpha-2-Macroglobulin antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver with Alpha-2-Macroglobulin antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Alpha-2-Macroglobulin antibody as confirmation of integrity and purity.