A-1210477

SKU: orb1305241

Description

A-1210477

Research Area

Cell Biology

Key Properties

• CAS Number: 1668553-26-1
• MW: 850.04
• Purity: 99.76%
• Formula: C₄₆H₅₅N₇O₇S
• SMILES: C(CN1CCOCC1)N2C3=C(C=CC=C3C(CCCOC=4C5=C(C=CC4)C=CC=C5)=C2C(O)=O)C6=C(COC7=CC=C(C=C7)N8CCN(S(N(C)C)(=O)=O)CC8)N(C)N=C6C
• Target: Bcl-2 Family,Apoptosis
• Solubility: DMSO:Insoluble;H2O:10 mM

Bioactivity

• Target IC50:
  Bcl-W:2280 nM (Ki)|Bcl-2:132 nM (Ki)|MCL1:26.2 nM|Bfl-1:660 nM (Ki)
• In Vitro:
  In H929 cells, A-1210477 binds to MCL-1 with high affinity and induces MCL-1 protein elevation. In H929, H2110, and H23 cells, A-1210477 induc the hallmarks of apoptosis, and inhibits MCL-1-dependent cell viability. A-1210477 also synergizes with navitoclax to kill a variety of cancer cell lines. In SKBR3 cells, A-1210477 inhibits MCL-1–BIM interaction and induces classical features of apoptosis. In addition, A-1210477 sensitizes non-Hodgkin's lymphoma cell lines to venetoclax (ABT-199).
• Cell Research:
  Adherent cell lines are seeded at 50,000 cells per well in 96-well plates and treated for 48 h with compounds diluted in half-log steps starting at 30 μM and ending at 0.001 μM. Multiple myeloma cell lines were seeded at 15,000–20,000 cells per well and treated similarly. Effects on proliferation and viability were determined using CellTiter-Glo reagent according to the manufacturer's instructions. IC50 Values are determined by non-linear regression analysis o the concentration response data.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Bcl-2 Family, A 1210477, A1210477, A-1210477, inhibit, Inhibitor, Mcl-1

Compound Identifiers

PubChem CID

66575373