6-TAMRA

SKU: orb1307237

Description

6-TAMRA

Research Area

Cell Biology

Key Properties

• CAS Number: 91809-67-5
• MW: 430.45
• Purity: 98.23%
• Formula: C₂₅H₂₂N₂O₅
• SMILES: CN(C)c1ccc2c(-c3cc(ccc3C([O-])=O)C(O)=O)c3ccc(cc3[o+]c2c1)N(C)C
• Target: Autophagy,Others
• Solubility: DMSO:2.86 mg/mL (6.64 mM)

Bioactivity

• In Vitro:
  6-TAMRA labeled nucleotide experiment Operation steps 1. Sample treatment: Incubate in NHOH/methylamine (1:1) solution at 65°C for 10 minutes to separate oligonucleotides from CPG. 2. Tak the supernatant and wash CPG with 1 mL EtOH/MeCN/H2O (3:1:1). 3. Mi the supernatants and dry them. 4. Treat with fresh anhydrous triethylammonium fluoride/n-methylpyridone (250 μL, n-methylpyridone 1.5 mL, triethylamine 750 μL, tea-3hf 1.0 mL) at 65°C for 1.5 h to remove t-butyl-dimethylsilyl protecting groups from RNA residues, and add 25 μL 3 M NaOAc and 1 mL n-BuOH to precipitate oligonucleotides. 5 the sample was cooled at -70°C for 1 h and then centrifuged at 10,000 g for 30 min the supernatant was decanted, washed with aqueous EtOH (70% v/v), and then dried. 6. 6-TAMRA ( 0.1 mL , 10 mg mL in dimethyl sulfoxide) was added to the 3'-amino-modified oligonucleotide suspended in 1.0 mL of sodium bicarbonate buffer (pH 8.5) and incubated at 37℃or 12 h. 7 the labeled oligonucleotide was resuspended in water and passed through a G25 Nap-10 disposable desalting column to remove free dye. 8 the oligonucleotide was purified by HPLC with a linear gradient of acetonitrile in 0.1 M triethylammonium acetate (TEAA) buffer, pH 7.2. 9 the entire sample was loaded on a Hamilton PRP-1 column and eluted with a linear gradient of acetonitrile for 40 min. Samples were monitored at 260 and 297 nM, and peaks corresponding to the dual-labeled oligonucleotide species were collected.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

6 TAMRA, 6TAMRA, 6-TAMRA, 6-Carboxytetramethylrhodamine, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2762604