6-TAMRA

SKU: orb1307237

Description

6-Carboxytetramethylrhodamine (6-TAMRA) is a widely used orange-red fluorophore for labeling biomolecules. It is essential for creating fluorescent probes and antibody conjugates applied in diverse techniques, including fluorescence microscopy, flow cytometry, and in vitro diagnostics, to study cellular processes.

Research Area

Cell Biology

Key Properties

• CAS Number: 91809-67-5
• MW: 430.45
• Purity: 98.23% (May vary between batches)
• Formula: C₂₅H₂₂N₂O₅
• SMILES: CN(C)c1ccc2c(-c3cc(ccc3C([O-])=O)C(O)=O)c3ccc(cc3[o+]c2c1)N(C)C
• Target: Others,Autophagy
• Solubility: DMSO:2.86 mg/mL (6.64 mM)

Bioactivity

• In Vitro:
  6-TAMRA labeled nucleotide experiment Operation steps 1. Sample treatment: Incubate in NHOH/methylamine (1:1) solution at 65°C for 10 minutes to separate oligonucleotides from CPG. 2. Take the supernatant and wash CPG with 1 mL EtOH/MeCN/H2O (3:1:1). 3. Mix the supernatants and dry them. 4. Treat with fresh anhydrous triethylammonium fluoride/n-methylpyridone (250 μL, n-methylpyridone 1.5 mL, triethylamine 750 μL, tea-3hf 1.0 mL) at 65°C for 1.5 h to remove t-butyl-dimethylsilyl protecting groups from RNA residues, and add 25 μL 3 M NaOAc and 1 mL n-BuOH to precipitate oligonucleotides. 5. The sample was cooled at -70°C for 1 h and then centrifuged at 10,000 g for 30 min. The supernatant was decanted, washed with aqueous EtOH (70% v/v), and then dried. 6. 6-TAMRA (0.1 mL, 10 mg/mL in dimethyl sulfoxide) was added to the 3'-amino-modified oligonucleotide suspended in 1.0 mL of sodium bicarbonate buffer (pH 8.5) and incubated at 37°C for 12 h. 7. The labeled oligonucleotide was resuspended in water and passed through a G25 Nap-10 disposable desalting column to remove free dye. 8. The oligonucleotide was purified by HPLC with a linear gradient of acetonitrile in 0.1 M triethylammonium acetate (TEAA) buffer, pH 7.2. 9. The entire sample was loaded on a Hamilton PRP-1 column and eluted with a linear gradient of acetonitrile for 40 min. Samples were monitored at 260 and 297 nm, and peaks corresponding to the dual-labeled oligonucleotide species were collected.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

6 TAMRA, 6TAMRA, 6-TAMRA, 6-Carboxytetramethylrhodamine, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2762604