Aminoallyl-dUTP (5/6-TAMRA)

• Catalog Number: orb64173
• Category: Tools
• Description: Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-5/6-TAMRA/dTTP ratio for Nick Translation: 35% Aminoallyl-dUTP-5/6-TAMRA/65% dTTP.
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: pink to red; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 935.66 g/mol (free acid); Detected MW: 935.16 g/mol (free acid)
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1cc(c(cc1)C(=O)[O-])c1c2c(oc3c1ccc(c3)N(C)C)cc(=[N+](C)C)cc2.C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1ccc(c(c1)C(=O)[O-])c1c2c(oc3c1ccc(c3)N(C)C)cc(=[N+](C)C)cc2
• Formula: C₃₇H₄₀N₅O₁₈P₃
• Storage: store at -20 °C
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λabs 545 nm, λem 575 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.