6-FAM SE

SKU: orb1307240

Description

6-FAM SE

Key Properties

• CAS Number: 92557-81-8
• MW: 473.39
• Purity: 98.23%
• Formula: C₂₅H₁₅NO₉
• SMILES: Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3ccc(cc23)C(=O)ON2C(=O)CCC2=O)c1
• Target: Others
• Solubility: 10% DMSO+40% PEG300+5% Tween-80+45% Saline:2 mg/mL (4.22 mM);DMSO:45 mg/mL (95.06 mM)

Bioactivity

• In Vitro:
  I. Nucleic acid sequencing 1. Labeling nucleotides: 6-FAM SE reacts with the 5' end of the nucleotide or oligonucleotide, and the succinic anhydride group reacts with the primary amine group in the nucleic acid to form a covalently bound fluorescent dye. 2. Fluorescence detection: During the sequencing process, the labeled nucleotides will emit fluorescence (excitation wavelength: 495 nm, emission wavelength: 520 nm), and the automatic sequencer can detect the fluorescence signal to determine the base sequence. II. Gene expression analysis 1. Labeling probes: 6-FAM is used as probes in qPCR experiments, which are designed to bind to specific regions of the target gene. 2. Quantification: The fluorescence signal generated during the amplification process is proportional to the number of target genes, so that gene expression can be quantitatively analyzed. III. Molecular biology detection: 1. Probe preparation: 6-FAM is labeled on a nucleic acid probe, which will hybridize with the target sequence. b. Detection: The fluorescence signal emitted by the 6-FAM-labeled probe is detected by fluorescence microscopy.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

6-Carboxyfluorescein N-succinimidyl ester, 6FAM SE, 6-FAM SE, 6 FAM SE, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2762614