5(6)-FAM SE

SKU: orb1307245

Description

5(6)-FAM SE

Key Properties

• CAS Number: 117548-22-8
• MW: 946.786
• Purity: 99.77%
• Formula: C₅₀H₃₀N₂O₁₈
• SMILES: Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3cc(ccc23)C(=O)ON2C(=O)CCC2=O)c1.Oc1ccc2c(Oc3cc(O)ccc3C22OC(=O)c3ccc(cc23)C(=O)ON2C(=O)CCC2=O)c1
• Target: Others
• Solubility: DMSO:120 mg/mL (126.74 mM)

Bioactivity

• In Vitro:
  Instructions 1. Solution preparation: Dissolve 5(6)-FAM SE in an appropriate solvent, such as anhydrous DMSO, DMF or methanol, to prepare a stock solution o the required concentration the recommended stock solution concentration is 10 mM for subsequent dilution. 2. Labeling process: a. dissolve the molecule to be labeled in an appropriate buffer. b. Add an appropriate amount of 5(6)-FAM SE stock solution to ensure tha the dye reacts fully wit the target molecule. c. Incubate at room temperature in the dark for 30 minutes to 1 hour to promot the labeling reaction. d. Afte the reaction is completed, was the labeled product with an appropriate buffer to remove unbound dye. 3. Fluorescence detection: Use a fluorescence microscope or flow cytometer for detection the excitation wavelength of 5(6)-FAM SE is approximately 495 nM, an the emission wavelength is approximately 517 nM. Precautions: a. Wear gloves during operation to avoid contact between skin or mucous membranes and reagents. b. Avoid light during incubation and storage to prevent fluorescence quenching. c. After staining is completed, fluorescence detection and analysis should be performed immediately.

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester, 5(6)-Carboxyfluorescein succinimidyl ester mixed isomers, 5(6) FAM SE, 5(6)FAM SE, 5(6)-FAM SE, Inhibitor, inhibit

Compound Identifiers

PubChem CID

131952919