5 Lipoxygenase Recombinant Rabbit Monoclonal Antibody

SKU: orb2563046

Description

5 Lipoxygenase Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 18A12
• Immunogen: A synthesized peptide derived from human 5 Lipoxygenase (120-155aa)
• Target: ALOX5
• Molecular Weight: 78 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Disclaimer: For research use only

Alternative Names

5-lipoxygenase; 5-LOX; 5 LO; 5LPG; ALOX 5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN.

25 ug total protein per lane of various lysates probed with 5 Lipoxygenase monoclonal antibody, unconjugated (orb2563046) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with 5 Lipoxygenase Monoclonal Antibody, Unconjugated (orb2563046) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

ALOX5

UniProt

P09917