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5-DBCO-PEG4-dUTP
Catalog Number: orb1734971
| Catalog Number | orb1734971 |
|---|---|
| Category | Small Molecules |
| Description | 5-DBCO-PEG 4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. |
| Form/Appearance | solution in 100 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
| Concentration | 10 mM-11 mM |
| Purity | ≥ 95% (HPLC) |
| MW | Theoretical MW: 1057.83 g/mol (free acid); Detected MW: 1057.25 g/mol (free acid) |
| Application notes | Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Formula | C42H54N5O21P3 |
| SMILES | c1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O |
| Storage | store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
5-DBCO-PEG4-dUTP [orb532299]
≥ 95% (HPLC)
Theoretical MW: 1057.83 g/mol (free acid); Detected MW: 1057.25 g/mol (free acid)
C42H54N5O21P3
5 x 50 μl
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
