5-DBCO-PEG4-dUTP

SKU: orb532299

Description

5-DBCO-PEG 4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: max 289 nm, 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 1057.83 g/mol (free acid); Detected MW: 1057.25 g/mol (free acid)
• Purity: 95% (HPLC)
• Formula: C₄₂H₅₄N₅O₂₁P₃
• SMILES: c1(=O)[nH]c(=O)n(cc1/C=C/CNC(=O)CCOCCOCCOCCOCCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only