360A

SKU: orb1299846

Description

360A is a small molecule that stabilizes G-quadruplex structures and inhibits telomerase activity with an IC50 of 300 nM in the TRAP-G4 assay. This compound is a valuable research tool for studying telomere biology and oncology, particularly in in vitro models of cancer cell proliferation and senescence.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 794458-56-3
• MW: 449.5
• Purity: 98.68% (May vary between batches)
• Formula: C₂₇H₂₃N₅O₂
• SMILES: C[N+]=1C2=C(C=C(NC(=O)C=3N=C(C(NC4=CC5=C([N+](C)=C4)C=CC=C5)=O)C=CC3)C1)C=CC=C2
• Target: Telomerase|||DNA/RNA Synthesis
• Solubility: DMSO:Slightly soluble

Bioactivity

• Target IC50:
  Telomerase:300 nM
• In Vitro:
  A549 lung carcinoma cell lines treatment with 5 μM of 360A led to an inhibition of cell proliferation within 11 days of treatmen. Treatment with the newly synthesized dimer (360A)2A led to a comparable antiproliferative effect
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• Cell Research:
  Supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin. A549 cells were maintained in humidified incubators at 37 °C in an atmosphere containing 5% CO2 and ambient oxygen of 20%. Cells were grown in 25 cm^2 flasks (150,000 cells/flask) and treated with 5 μM of 360A or (360A)2A every 3 or 4 days (i.e., at each reseeding) until cultures were terminated. Control cells were either non-treated or treated with 0.1% DMSO. At each reseeding the remaining cells were pelleted and snap frozen for further DNA extraction.Statistical analysis was performed using GraphPad Prism 5. A Chi-square test was used to compare TDEs frequencies.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA Synthesis, DNASynthesis, 360A, G-quadruplex, Inhibitor, inhibit, RNASynthesis, RNA Synthesis, Telomerase

Compound Identifiers

PubChem CID

11430774