2-hydroxyisobutyryl-HIST1H4A (K16) antibody

• Catalog Number: orb516315
• Category: Antibodies
• Description: 2-hydroxyisobutyryl-HIST1H4A (K16) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide sequence around site of 2-hydroxyisobutyryl-Lys (16) derived from Human Histone H4
• Dilution range: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIST1H4A
• UniProt ID: P62805
• Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A or a control normal rabbit IgG. The resulting ChIP DNA was quantified usi

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysateusing 2-hydroxyisobutyryl-HIST1H4A (K16) antibody

Immunoprecipitation analysis of HepG2 whole cell lysate using 2-hydroxyisobutyryl-HIST1H4A (K16) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) using 2-hydroxyisobutyryl-HIST1H4A (K16) antibody