2-hydroxyisobutyryl-HIST1H4A (K16) Antibody

• Catalog Number: orb516315
• Category: Antibodies
• Description: This 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody is an unconjugated polyclonal product. It tagets HIST1H4A using a Peptide sequence around site of 2-hydroxyisobutyryl-Lys (16) derived from Human Histone H4 as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IP, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H4A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of 2-hydroxyisobutyryl-Lys (16) derived from Human Histone H4
• UniProt ID: P62805
• Tested applications: ChIP, ELISA, IF, IP, WB
• Dilution range: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: dJ160A22.1 antibody; dJ160A22.2 antibody; dJ221C16
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H4A or a control normal rabbit IgG. The resulting ChIP DNA was quantified usi

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, A549 whole cell lysateusing 2-hydroxyisobutyryl-HIST1H4A (K16) antibody

Immunoprecipitation analysis of HepG2 whole cell lysate using 2-hydroxyisobutyryl-HIST1H4A (K16) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) using 2-hydroxyisobutyryl-HIST1H4A (K16) antibody