1,4-Chrysenequinone

SKU: orb1940848

Description

1,4-Chrysenequinone is an activator of aryl hydrocarbon receptor (AhR).(In Vitro):Mouse hepatoma (H1L1.1c2) cells (1.5 × 105 cells/well) are cultured in 96-well culture plates, and samples (1,4-Chrysenequinone) dissolved in dimethyl sulfoxide are added to the medium to achieve a final solvent concentration of 1%. After the plates are incubated at 37°C in 5% CO2 for 24 h, the cell viability is confirmed under a microscope. Subsequently, the medium is removed, and the cells are lysed. Adding luciferin as the substrate, the luciferase activity is determined under a luminometer and reported as relative light units. The concentrations producing luciferase activity equal to 25% of the maximal response to TCDD are calculated and also referred to as the ECTCDD25. The ratios of the ECTCDD25 of B[a]P to the ECTCDD25 of each of the compounds are calculated and referred to as IEFs. 1,4-Chrysenequinone shows AhR ligand activity, with ECTCDD25s (concentration equivalent with 25% of TCDD max) of 9.7 nM and 1.9 μM in yeast and mouse hepatoma cell systems, respectively.

Key Properties

• CAS Number: 100900-16-1
• MW: 258.276
• Purity: >98% (HPLC)
• Formula: C₁₈H₁₀O₂
• SMILES: O=C1C=CC(=O)c2c1ccc1c2ccc2ccccc12
• Target: AhR
• Solubility: In Vitro: DMSO : 3.33 mg/mL (12.89 mM)

Bioactivity

• In Vitro:
  1, 4-Chrysenequinone shows significant AhR ligand activity, with ECTCDD25s (concentration equivalent with 25% of TCDD max) of 9.7 nM and 1.9 μM in yeast and mouse hepatoma cell systems, respectively.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chrysene-1,4-dione

Quick Database Links

Gene Symbol

AhR