YWHAZ Antibody

SKU: orb1253752

Description

YWHAZ Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: 14-3-3 zeta (Phospho-Ser58) antibody was raised against a peptide sequence around phosphorylation site of serine 58 (R-S-S (p) -W-R) derived from Human 14-3-3 zeta.
• Target: YWHAZ
• Molecular Weight: 28 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HEL4, YWHAD, KCIP-1, HEL-S-3, 14-3-3-zeta, JNK2, PRKM9, SAPK1A, 1433Z, 143Z, FAS, Factor activating exoenzyme S

Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using 14-3-3 zeta (Phospho-Ser58).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (Phospho-Ser58). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using 14-3-3 zeta (Phospho-Ser58).

Quick Database Links

Gene Symbol

YWHAZ

UniProt

P63104

RefSeq (Protein):NP_001129171.1