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14-3-3 Tau Recombinant Rabbit Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 2H12 |
| Immunogen | A synthesized peptide derived from human 14-3-3 theta (1-48aa) |
| Target | YWHAQ |
| Molecular Weight | 28 kDa |
| Purification | Affinity purified by Protein A |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), (HRP), Lysate: IMR-32, 2: Caco-2, 3: Mouse kidney, 4: Rat testis, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 28 kDa, Observed MW: 28 kDa.
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Protocol Information
14-3-3 Tau Recombinant Rabbit Monoclonal Antibody (orb559067)
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