14-3-3 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172936
• Category: Antibodies
• Description: 14-3-3 Recombinant Mouse Monoclonal Antibody
• Target: YWHAZ
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: IgG1/Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Tested applications: ICC, WB
• Dilution range: WB=1:200-1000, ICC/IF=1:20-100
• Antibody Type: Recombinant Antibody
• Clone Number: B12C1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: pan 14-3-3; 14 3 3 protein beta; 14 3 3 protein be
• Note: For research use only

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: A549, 3: Neuro-2a, 4: PC-12, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 28 kDa, Observed MW: 29 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% Triton X-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172936.