ZNF133 Rabbit Polyclonal Antibody

SKU: orb576628

Description

Rabbit polyclonal antibody to ZNF133

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZNF133
• Target: ZNF133
• Molecular Weight: 73kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

ZNF150, pHZ-13, pHZ-66

Three cryosections on each slide were stained with a single antibody. The fourth slice was used as a negative control (primary antibody omitted). The tissue was fixed with cold acetone for 10 minutes, rinsed in phosphate buffered saline (PBS) and incubated with the primary antibody diluted in 1% bovine serum albumin (BSA) in PBS for 1 hour at room temperature. Rabbit anti human antibodies and dilutions used:e) ZNF133 (38332), most suitable concentration 1:100.

WB Suggested Anti-ZNF133 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: THP-1 cell lysate.

Quick Database Links

Gene Symbol

ZNF133

UniProt

Q53XU1

RefSeq (Protein):NP_003425