Zebrafish Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU: orb3127196

Description

The Zebrafish Atrial Natriuretic Peptide (ANP) ELISA Kit is designed to quantify Atrial Natriuretic Peptide (ANP) in zebrafish serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 4.60 pg/mL.

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish ANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish ANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish ANP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Zebrafish
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 15.63-1000 pg/mL
• Sensitivity: 4.60 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin

Quick Database Links

Gene Symbol

ANP