XMD17-109

SKU: orb1307343

Description

XMD17-109

Research Area

Signal Transduction

Key Properties

• CAS Number: 1435488-37-1
• MW: 638.8
• Purity: 99.75%
• Formula: C₃₆H₄₆N₈O₃
• SMILES: CCOc1cc(ccc1Nc1ncc2N(C)C(=O)c3ccccc3N(C3CCCC3)c2n1)C(=O)N1CCC(CC1)N1CCN(C)CC1
• Target: ERK
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.17 mM);DMSO:93 mg/mL (145.59 mM);Ethanol:93 mg/mL (145.59 mM)

Bioactivity

• Target IC50:
  ERK5:162 nM(EC50)|LRRK2 (G2019S):339 nM
• In Vivo:
  At the cellular level, ERK5-IN-1 inhibits endothelial growth factor-induced ERK5 autophosphorylation (EC50: 90 nM). It also effectively inhibits the biochemical properties of ERK5 (IC50: 162 nM).
• Cell Research:
  HeLa cells are maintained in DMEM supplemented with 10% FBS, 2 mM l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. Before use HeLa cells are serum starved for 16 h in DMEM supplemented with 2 mM l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. HeLa cells are then incubated with ERK5-IN-1 at the indicated concentrations for 1 h prior to stimulation with 0.5mol/Lsorbitol for 30 min. Cells are lysed in Triton lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EGTA, 1 mM EDTA, 1 mM sodium orthovanadate, 50 mM sodium fluoride, 1 mM sodium pyrophosphate, 0.27mol/Lsucrose, 1 μM microcystin-LR, 1% (v/v) Triton X-100, 0.1% (v/v) 2-mercaptoethanol) and 20 μg of protein loaded per well. Samples are run on 8% polyacrylamide gels using standard methods. Proteins are transferred onto nitrocellulose membranes and specific proteins detected by immunoblotting.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Extracellular signal regulated kinases, ERK, ERK5-IN-1, ERK5, Inhibitor, inhibit, XMD17 109, XMD17109, XMD-17-109, XMD17-109

Compound Identifiers

PubChem CID

71604307