XI-006

SKU: orb1300617

Description

XI-006 is a small molecule inhibitor of MDMX (IC50 = 2.5 µM) and modulator of MDM2, which promotes p53 stabilization and activation. This compound is a useful research tool for studying p53 pathway biology, DNA damage response, and cancer mechanisms in both cellular and biochemical assays.

Research Area

Cell Biology

Key Properties

• CAS Number: 58131-57-0
• MW: 279.25
• Purity: 99.12%
• Formula: C₁₁H₁₃N₅O₄
• SMILES: CN1CCN(CC1)c1ccc([N+]([O-])=O)c2no[n+]([O-])c12
• Target: p53,Mdm2,MDM-2/p53
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.16 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:55 mg/mL (196.96 mM)

Bioactivity

• Target IC50:
  MDMX:2.5 μM
• In Vitro:
  XI-006 decreases both the MDMX mRNA and protein in MCF-7 cells. XI-006 induces expression of p53 as well as well-characterized p53-target gene, p21 and MDM2, in a dose-dependent manner in MCF-7 cells. XI-006 extends the half-life of p53 from 20 to 30 minutes to more than 3 hours as revealed by cycloheximide chase assays in MCF-7 cells. XI-006 also activates p53 and induces p21 and MDM2 expression in LNCaP prostate and A549 lung cancer cells. XI-006 increases the mRNA levels of proapoptotic genes including PUMA, BAX, and PIG3 in a dose-dependent manner in MCF-7 cells. XI-006 results in a significant increase in the numbers of sub-G0/G1 cells as well as G2 arrest. XI-006 also results in more than 40% of cells dying via apoptosis and decreases cell viability in A549 and LNCaP cells. XI-006 inhibits biosynthesis of nucleic acids and proteins in L1210 cells. XI-006 interacts with DNA repair to activate the DNA damage repair pathway in three species (S. cerevisiae, S. pombe and H. sapiens). XI-006 acts as cytotoxic agent in the G/R-luc astrocytoma cell line with GI50 of 117 nM.
• Cell Research:
  MCF-7 cells treated with dimethyl sulfoxide (DMSO), nutlin-3a, or NSC-207895 are permeabilized with cold 70% ethanol overnight, and stained with a solution containing 50 μg/mL propidium iodide and 20 μg/mL RNase A at 37°C for 20 minutes. The cells are then subjected to flow cytometry analysis. The FlowJo software is used to calculate percentages of cells in each cell cycle phase. For terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL) staining, MCF-7 cells treated with the NSC-207895 for 2 days are fixed with 4% paraformadelhyde for 1 hour, and then subjected to dUTP labeling using In Situ Cell Death Detection Kit TMR Red according to the manufacturer's protocol. For quantitation, at least 300 cells are randomly chosen and the numbers of TUNEL-positive cells are counted. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, MDMX, MDM2, MDM-2/p53, inhibit, LPS, NSC 207895, NSC207895, NSC-207895, p53, XI 006, XI006

Compound Identifiers

PubChem CID

42640