WS-383

SKU: orb1308938

Description

WS-383 is a potent and selective small molecule inhibitor of the DCN1-UBC12 protein-protein interaction, exhibiting reversible binding with an IC50 of 11 nM. It serves as a valuable chemical probe for studying cullin neddylation in biochemical and cellular assays, with utility in cancer and developmental biology research.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 2247544-02-9
• MW: 498.46
• Purity: 98.80% (May vary between batches)
• Formula: C₁₈H₂₁Cl₂N₉S₂
• SMILES: Cl.CN(C)CCn1nnnc1Sc1cc(C)nc2nc(SCc3ccc(Cl)cc3)nn12
• Target: E1/E2/E3 Enzyme
• Solubility: DMSO:6.5 mg/mL (13.04 mM)

Bioactivity

• Target IC50:
  DCN1-UBC12:11 nM
• In Vitro:
  WS-383 (10 μM) is selective to the DCN1–UBC12 interaction over the selected kinasesr. WS-383 (0.03-3 μM;24 hours) blocks Cul3 neddylation at 3 μM and also has certain inhibition of Cul1 neddylation at 10 μM but was not effective in inhibiting neddylation of other cullin members. WS-383 (0.03-3 μM;24 hours) increases Cul1, Skp1 (adaptor protein), F-box protein, and RBX1/RBX2 RING protein form SCF E3 complex. Cyclin dependent kinase inhibitor 1A (p21) and cyclin dependent kinase inhibitor 1B (p27) expression in a dose-dependent manner in MGC-803 and KYSE70 manner.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell, DCN1-UBC12, DCN1-UBC12 interaction, E2Enzyme, E3 Enzyme, E2 Enzyme, E2 conjugating enzyme, E1Enzyme, E1 activating enzyme, E1 Enzyme, E1/E2/E3 Enzyme, E3 ligating enzyme, E3Enzyme, Inhibitor, interaction, KYSE70, inhibit, MGC-803, WS383, WS-383, WS 383, Ubiquitin ligase, Ubiquitin activating enzyme, Ubiquitin conjugating enzyme

Compound Identifiers

PubChem CID

138319703