WS-12

SKU: orb1220428

Description

WS-12 is a potent TRPM8 agonist that acts as a cooling agent (EC50 : 193 nM).

Key Properties

• CAS Number: 68489-09-8
• MW: 289.41
• Purity: >98% (HPLC)
• Formula: C₁₈H₂₇NO₂
• SMILES: COc1ccc(NC(=O)[C@@H]2C[C@H](C)CC[C@H]2C(C)C)cc1
• Target: TRP/TRPV Channel
• Solubility: DMSO:20 mg/mL (69.11 mM)

Bioactivity

• In Vivo:
  Acoltremon (intraperitoneal injection; 10 mg/kg; once) shows analgesic effect by selective TRPM8 activation in mice. Animal model: Trpm8–/– mice and mice injected with Capsaicin. Dosage: 10 mg/kg. Administration: Intraperitoneal injection; 10 mg/kg; once. Result: Produced an obvious analgesic effect, and this effect abolished in Trpm8–/– mice. Showed no change falling latencies in mice in the rotarod test. Reduced Capsaicin-induced nocifensive behavior.
• In Vitro:
  Acoltremon (10 μM) shows TRPM8 agonism in tests on heterologously expressed TRP ion channels. Acoltremon (1 or 10 μM; 0-250 s) shows highly potent and specific TRPM8 channel agonist in DRG neurons. Cell Viability Assay Cell line: HEK293T cells. Concentration: 10 μM. Incubation time: Result: Activated mouse TRPM8 (mTRPM8), but neither activated nor inhibited mTRPA1.Cell Viability Assay Cell line: Cultured mouse DRG neurons. Concentration: 1 or 10 μM. Incubation time: 0-250 s. Result: Showed approximately 10% to 14% of cultured wild-type neurons responsive to WS-12.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

TRP/TRPV Channel