WAY-600

SKU: orb1685620

Description

WAY-600 is a potent and selective ATP-competitive mTOR inhibitor with an IC50 of 9 nM. It effectively suppresses both mTORC1 and mTORC2 signaling by inhibiting phosphorylation of S6K(T389) and AKT(S473), respectively, and demonstrates high selectivity over PI3K isoforms. This inhibitor is a valuable tool for studying mTOR-dependent pathways in cancer and metabolic disease research, applicable in both cellular and in vivo models.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 1062159-35-6
• MW: 494.59
• Purity: 99.88% (May vary between batches)
• Formula: C₂₈H₃₀N₈O
• SMILES: C(N1CCC(CC1)n1ncc2c(nc(nc12)-c1ccc2[nH]ccc2c1)N1CCOCC1)c1cccnc1
• Target: Src,mTOR,PI3K,VEGFR
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.04 mM);Ethanol:2 mg/mL (4.04 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:45 mg/mL (90.98 mM)

Bioactivity

• Target IC50:
  PI3Kα:1.96 μM|mTOR:9 nM|PI3Kγ:8.45 μM
• In Vitro:
  Immune-complex kinase assay shows mTORC2-specific substrate His6-AKT or the mTORC1 substrate His6-S6K is dose dependently inhibited by WAY-600. WAY-600 globally inhibits mTOR signaling and AKT function in cellular models dose dependently. WAY-600 profoundly inhibits cap-dependent and global protein synthesis in MDA361, human breast cancer cells. WAY-600 shows antiproliferative effects in various cancer cell lines involving G1 cell cycle arrest and selective apoptosis. WAY-600 down-regulate angiogenic factors, VEGF and HIF-1α, in U87 mg, MDA361 and LNCap cells.
• Cell Research:
  For cell cycle analysis, cells are seeded in 96-well culture plates at 10,000 cells per well for 24 hours, treated with various inhibitors for 24 hours or 48 hours. Following treatment, cells are harvested, washed with PBS and fixed overnight at -20 °C in 70% ethanol. Cells are washed, stained with propidium iodide and analyzed for cell cycle profile (acquired 5000 cells/well) on Guava PCA-96 instrument according to the Guava Cell Cycle Protocol(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mammalian target of Rapamycin, Inhibitor, mTOR, inhibit, Src, WAY 600, PI3Kγ, PI3Kα, WAY600, WAY-600, VEGFR2

Compound Identifiers

PubChem CID

25229526