WAY-100635 maleate

SKU: orb1695294

Description

WAY-100635 Maleate is a potent and selective 5-HT1A receptor antagonist with an IC50 of 0.95 nM. It is widely used in neuroscience research to study serotonin signaling in both in vitro binding assays and in vivo behavioral models.

Research Area

Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 634908-75-1
• MW: 538.645
• Purity: >99.99% (May vary between batches)
• Formula: C₂₅H₃₄N₄O₂.xC₄H₄O₄
• SMILES: OC(=O)C=CC(O)=O.COc1ccccc1N1CCN(CCN(C(=O)C2CCCCC2)c2ccccn2)CC1
• Target: Dopamine Receptor,5-HT Receptor
• Solubility: Ethanol:79 mg/mL;DMSO:79 mg/mL

Bioactivity

• Target IC50:
  sPLA2:8.87 (pIC50)|sPLA2:9.71 (pA2)
• Cell Research:
  Extracellular recordings are made with glass microelectrodes filled with 2 M NaC1 (12 MΩ-15 MΩ). Cells are identified as 5-HT neurons according to the following criteria: biphasic action potentials of 2 msec to 3 msec in duration, slow (0.5 Hz - 2.0 Hz) and regular pattern of discharge. Firing is evoked in the otherwise silent neurons by adding the alpha-l adrenergic agonist phenylephrine (3 μM) to the superfusing ACSF. Baseline activity is recorded for at least 10 minutes before application of the different drugs. The electric signals are fed into a high-input impedance amplifier, an oscilloscope and an electronic ratemeter triggered by individual action potentials connected to an A/D converter and a personal computer. Using dedicated software, the integrated firing rate is recorded, computed and displayed on a chart recorder as consecutive 10-sec samples. The effects of agonists are evaluated by comparing the mean discharge frequency recorded during the 2 minutes that preceded WAY 100635 application with that recorded at the peak of WAY 100635 action (usually 2-5 minutes after the beginning of application). When the agonists are applied in the presence of the antagonist, the effect of the agonist is compared to baseline firing rate and to the frequency recorded during superfusion of the antagonist alone. The antagonist is left to equilibrate for 10 minutes to 25 minutes before retesting of the action of agonists. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5HT Receptor, 5HTReceptor, WAY100635 Maleate, WAY100635 maleate, WAY-100635 Maleate, WAY 100635 maleate, WAY 100635 Maleate

Compound Identifiers

PubChem CID

57351886