VU0359595

SKU: orb1704970

Description

VU0359595 (ML-270) is a highly selective phospholipase D1 (PLD1) inhibitor with a 3.7 nM IC50 and >1700-fold selectivity over PLD2. This tool compound is used in research areas including cancer, inflammation, and neurodegeneration, facilitating both in vitro mechanistic studies and in vivo disease model investigations.

Research Area

Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 1246303-14-9
• MW: 497.43
• Purity: 99.79% (May vary between batches)
• Formula: C₂₅H₂₉BrN₄O₂
• SMILES: C[C@@H](CN1CCC(CC1)n1c2ccc(Br)cc2[nH]c1=O)NC(=O)[C@@H]1C[C@H]1c1ccccc1
• Target: Phospholipase,Antifungal
• Solubility: DMSO:4.98 mg/mL (10.01 mM)

Bioactivity

• Target IC50:
  PLD1:3.7 nM|PLD2:6.4 μM
• In Vitro:
  VU0359595, at concentrations ranging from 5 to 5000 nM, inhibits both the baseline and FCS/IGF-1 stimulated proliferation of astroglial cells. At doses of 5, 50, and 500 nM, with a 30-min exposure, it does not impact the basal phospholipase D (PLD) activity in astrocytes; however, it significantly reduces mitogen-stimulated PLD activity in a concentration-dependent manner. Additionally, at a concentration of 0.15 μM administered 1 h before and during a 4 h high glucose treatment, VU0359595 notably decreases the high glucose-induced increase in [3H]-phosphatidylethanol (PEth) generation in retinal pigment epithelium (RPE) cells. When applied at 5 μM one hour before lipopolysaccharide (LPS) treatment, it modulates autophagy in LPS-induced RPE cells undergoing a 24-h treatment with 10 μg/ml of LPS. Furthermore, a 2 nM pre-exposure for 30 min effectively blocks the gliotoxin (50 ng/ml)-enhanced internalization of A. fumigatus in A549 cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VU 0359595, VU0359595, VU-0359595, VU0359595-1, ML 270, ML270, ML-270, PLD1, CID 53361951, CID53361951, CID-53361951

Compound Identifiers

PubChem CID

44573131