VPS35 ELISA Kit

SKU: orb1822517

Description

Colorimetric measurement of VPS35

Research Area

Neuroscience

Images & Validation

• Application Notes:
  1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at 37°C for 1 hour shaking at 600rpm. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes, shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.

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Key Properties

• Reactivity: Human, Mouse, Rat
• Sample Types: Tissue, Cell Lysate
• Assay Type: Sandwich
• Range: 78.125 - 5000 pg/mL
• Sensitivity: 50 pg/mL

Storage & Handling

• Storage: 4°C and -20°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FLJ10752, MEM3, PARK17, TCCCTA00141, VPS35 retromer complex component, Vacuolar protein sorting-associated protein 35, maternal-embryonic 3, vesicle protein sortin 35

Quick Database Links

Gene Symbol

VPS35 ELISA Kit