VO-Ohpic trihydrate

SKU: orb1300145

Description

VO-Ohpic trihydrate

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 476310-60-8
• MW: 415.2
• Purity: >99.99%
• Formula: C₁₂H₉N₂O₈V·3H₂O·H
• SMILES: O=C1O[V+](=O)(Oc2c1nccc2)OC(=O)c1ncccc1O.O.O.O.O.[H-]
• Target: Autophagy,PTEN
• Solubility: DMSO:16.67 mg/mL (40.15 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.41 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  PTEN:35 nM
• In Vivo:
  VO-Ohpic significantly inhibits tumor growth in nude mice bearing xenografts of Hep3B cells. VO-Ohpic administered to C57BL6 mice 30 minutes prior to Kcl-induced asystolic cardiac arrest significantly increases survival, LVPmax and dP/dt max with continued benefit. VO-OHpic also significantly increases lactate clearance and decreases plasma glucose level.
• In Vitro:
  VO-OHpic is a potent small-molecule compound that specifically inhibits PTEN's cellular enzymatic activity, which in turn activates downstream targets such as Akt and FoxO3a. Glucose uptake into adipocytes is dramatically enhanced upon PTEN inhibition with VO-OHpic. PTEN inhibitor accelerates wound healing in fibroblasts. VO-OHpic inhibits cell viability, cell proliferation and colony formation, and induces senescence-associated β-galactosidase activity in Hep3B (low PTEN expression) and to a lesser extent in PLC/PRF/5 (high PTEN expression) cells, but not in PTEN-negative SNU475 cells.
• Cell Research:
  Cell proliferation is determined by estimating the amount of bromodeoxyuridine (BrdU) incorporation into DNA by a colorimetric immunoassay. 3×103 cells are cultured in 96-well plates with varying concentrations of VO-OHpic for 72 hours. BrdU is added 24 hours before the end of the treatments. Results are expressed as the percentage inhibition of BrdU incorporation over the control.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VOOhpic trihydrate, VO-Ohpic Trihydrate, VO Ohpic trihydrate, PTEN, Phosphatase and tensin homolog, Autophagy, MMAC1

Compound Identifiers

PubChem CID

137247305