Vistusertib

SKU: orb1306966

Description

Vistusertib (AZD2014) is an orally active mTOR inhibitor with potential anticancer properties. It is used in preclinical research to study mTOR signaling in oncology, demonstrating activity in both in vitro cell-based assays and in vivo tumor models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 1009298-59-2
• MW: 462.54
• Purity: 99.06%
• Formula: C₂₅H₃₀N₆O₃
• SMILES: CNC(=O)c1cccc(c1)-c1ccc2c(nc(nc2n1)N1CCOC[C@@H]1C)N1CCOC[C@@H]1C
• Target: Akt,S6 Kinase,mTOR,Apoptosis,Autophagy,PI3K
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.32 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:36 mg/mL (77.83 mM)

Bioactivity

• Target IC50:
  mTOR:2.8 nM|pS6 (S235/236):200 nM|PI3Kα:3.766 μM|pAkt (S473):80 nM
• In Vivo:
  In hormone therapy-responsive ER+ breast cancer cell lines, AZD2014 inhibits cellular growth and promotes apoptosis. It effectively suppresses the biomarkers of mTORC2, namely pAKTSer473 and pNDRG1Thr346.
• In Vitro:
  In the human ER+ breast cancer xenograft model, AZD2014 inhibits tumor growth by regulating the substrates of mTORC1 and mTORC2.
• Cell Research:
  AZD2014 is prepared in DMSO (10 mM) and stored under nitrogen, and then diluted with appropriate media before use. Cells are plated in 96-well plates for the indicated time. For CellTiterGlo assays: CellTiterGlo is mixed with the cells. Cells are normalized to day 0 control and net growth is determined using the following formula: ((x y)/(z y))=net growth, where x=reading of treated sample at end of study, y=average reading on day 0, and z=reading of DMSO-treated sample at end of study. The concentration of DMSO does not exceed 0.03% for any experiment. For MTS assays: adherent cell lines are grown in 96-well plates. MTS reagent is added on day 0 and on day 3 post-AZD2014 addition. Suspension lines are assayed using the Alamar Blue reagent, 72 hours after AZD2014 addition.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AZD 2014, AZD2014, AZD-2014, Autophagy, Apoptosis, Mammalian target of Rapamycin, Inhibitor, P-Akt (S473), inhibit, mTOR, pS6 (S235/236), S6 Kinase, S6Kinase, PI3Kα, Vistusertib

Compound Identifiers

PubChem CID

25262792