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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Immobilized Human VEGF165 (1 ng/well) can bind Human VEGF R1, His Tag with a linear range of 4-30 ng/mL. |
| Target | VEGFA |
| Tag | Tag free |
| MW | 19 kDa (monomer) |
| Purity | >98% as determined by SDS-PAGE and more than 95% rhVEGF165 exists as active homodimer in analytical SEC-HPLC assay. |
| Protein Sequence | Ala 27 - Arg 191 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Human VEGF165, Tag Free (HPLC-verified) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001165097 |
|---|
Documents Download
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Protocol Information
VEGF 165 Recombinant Protein (orb1227834)
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