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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Immobilized Human VEGF165, His Tag at 2ug/mL (100uL/well) can bind VEGFR1/R2-Fc with a linear range of 0.1-3 ng/mL. |
| Target | VEGFA |
| Tag | His Tag |
| MW | 21.1 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Ala 27 - Arg 191 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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The purity of rh VEGF165 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
VEGF 165 Recombinant Protein (orb1227833)
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