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Catalog Number | N417-01 |
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Category | Tools |
Description | The Ribo-off rRNA Depletion Kit V2 (Bacteria) is designed to remove ribosomal RNA (rRNA) from the total RNA of gram-positive and gram-negative bacteria. This kit is compatible with an initial template input of 0.01 - 1 μg. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, and other steps for the rRNAs (including 16S and 23S rRNA) to be depleted while retaining mRNA and other non-coding RNA. This kit is applicable to both intact and partially degraded RNA samples, and the resulting RNA can be used for the analysis of mRNA and non-coding RNA (such as LncRNA). The optimized reaction system enhances the depletion effect and shortens the workflow time. |
Storage | Store at -30 ~-15°C and transport at < 0°C. |
Alternative names | rRNA Depletion;RNA Library Prep;Bacteria;N417 Read more... |
Background | Only available for the UK and Republic of Ireland |
Note | For research use only |
Application notes | Coexistence of speed and yield: optimize the system to ensure rRNA removal efficiency and shorten the process to 1h. Wide range of application: The coverage of species is improved, and the applicable species are wider. |
Expiration Date | 12 months from date of receipt. |