Vazyme - 2 × Phanta Flash Master Mix

SKU: P510-03

Description

2 × Phanta Flash Master Mix is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Throughdirected optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. The amplification error rate of Phanta Flash Super-Fidelity DNA Polymerase is 81-foldlower than that of conventional Taq DNA Polymerase. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains twotypes of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling itto perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase,dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detectionthroughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (Vazyme#C112/C113/C116) and TOPO cloning kit (Vazyme #C603).

Images & Validation

• Application Notes:
  High fidelity: ~81X higher than Taq DNA Polymerase Flash amplification: Extension speed up to 5 sec/kb Robust performance: Superior specificity, sensitivity, and yields Broad compatibility: Suitable for complex samples & GC-rich systems

Storage & Handling

• Storage: Store at -30 ~ -15°C and transport at ≤0°C.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Vazyme - 2 × Phanta Flash Master Mix, 2 x Phanta Flash Master Mix (Dye Plus) (Vazyme #P520) and other commercially available brands of high-fidelity polymerases were used to perform PCR amplification. The target fragment of 353 bpcially available brands of high-fidelity polymerases were us7140 bp fragment was amplified at an extension time of 4 sec/kband 873 bp were amplified at an extension time of 1 sec/kb and 5 sec/kb, respectively. The results show that all the frag ments could be successfully amplified by P510 and P520.

Amplification of fragments ≤10 kb can be achieved within 1 h.

Vazyme #P510, Vazyme #P520 and other commercially available brands of high-fidelity polymerases were used to amplifydifferent length of fragments in accordance to the recommended systems and procedures of respective instructions. The results show that P510 and P520 have higher amplification specificity than other brands of high-fidelity polymerases.