This compound is a highly selective and potent agonist for PSAM4-GlyR and PSAM4-5-HT3 chimeric ion channels, with EC50 values of 0.3 nM and 0.5 nM, respectively. It effectively silences targeted neuronal activity in brain slices and induces robust rotational behavior in vivo in mouse models, making it a powerful tool for precise chemogenetic control of neuronal circuits.