UNC1215

SKU: orb1305570

Description

UNC1215 is a potent and selective chemical probe targeting the methyl-lysine reader protein L3MBTL3 with high affinity (Kd 120 nM) and 50-fold selectivity over other MBT family members. It is a valuable tool for investigating chromatin signaling and gene regulation in both biochemical and cellular studies.

Research Area

Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 1415800-43-9
• MW: 529.72
• Purity: 98.87%
• Formula: C₃₂H₄₃N₅O₂
• SMILES: O=C(N1CCC(CC1)N1CCCC1)c1ccc(C(=O)N2CCC(CC2)N2CCCC2)c(Nc2ccccc2)c1
• Target: Epigenetic Reader Domain,Histone Methyltransferase,Apoptosis
• Solubility: DMSO:93 mg/mL (175.56 mM);Ethanol:93 mg/mL (175.56 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween-80+45% Saline:1 mg/mL (1.89 mM)

Bioactivity

• Target IC50:
  L3MBTL3:120 nM(Kd)|L3MBTL3:40 nM
• In Vivo:
  UNC1215 binds to L3MBTL3 and competitively displaces peptides containing mono- or dimethyl-lysine.UNC1215 is approximately 75-fold more selective for L3MBTL3 than L3MBTL1.UNC1215 is non-cytotoxic and binds directly to L3MBTL3 through the Kme-binding pocket of the MBT domain.UNC1215 enables the cellular mobility of GFP-L3MBTL3 fusion proteins and the cellular mobility of the fusion protein. UNC1215 increased cell mobility and point mutations of the GFP-L3MBTL3 fusion protein, interfered with the Kme-binding function of the GFP-L3MBTL3 phenotypic mimic, and affected the localization of UNC1215.UNC1215 (30 μM) did not affect the tandem Tudor domain of UHRF1, the chromatin domain of CBX7, and the PHD domain of JARID1A.
• Cell Research:
  CellTiter-Glo luminescent cell viability assay(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Cerebrum, inhibit, methyllysine, Inhibitor, Malignant, MBT, L3MBTL3, L3MBTL3-D274A, Histone Methyltransferase, HistoneMethyltransferase, Epigenetic Reader Domain, EpigeneticReaderDomain, UNC 1215, UNC1215, UNC-1215, probe

Compound Identifiers

PubChem CID

57339144