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TWEAK Recombinant Protein
Description
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Assay #1: The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/mL.Assay #2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-gamma. The ED50 for this effect is between 30-45 ng/mL. |
| Target | TNFSF12 |
| Protein Sequence | MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses. |
Storage & Handling
−| Storage | The lyophilized TWEAK recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted TWEAK is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing TWEAK recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human TWEAK R [orb2993998]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 32.7 KDa. Observed: 30-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgMouse TWEAK R [orb2993966]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 32.6 KDa. Observed: 30-40 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman TNFSF12 Protein, hFc Tag [orb757421]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 43.3 kDa after removal of the signal peptide.
Mammalian
50 μg, 10 μg, 100 μg

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Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003800.1 |
|---|
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Protocol Information
TWEAK Recombinant Protein (orb1230505)
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