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p53 Antibody Human Research
Application-Focused Human p53 and TP53 Antibodies for Cancer Biology, DNA Damage Response, WB, IHC, IF, FC, IP and ChIP Workflows.
1. Target Overview: p53, TP53 & Human Cancer Biology
p53, encoded by the TP53 gene, is one of the most important tumour suppressor proteins in human cancer research. It functions as a transcription factor that responds to DNA damage, oncogenic stress, hypoxia and other cellular signals that threaten genomic stability.
In human cells and tumour samples, p53 regulates key biological outcomes including cell cycle arrest, apoptosis, senescence, DNA repair and metabolic adaptation. Because TP53 is frequently altered in cancer, a validated p53 antibody human workflow is essential for studying tumour progression, treatment response, pathway activation and biomarker development.
Research Relevance in Oncology: Human p53 studies often require more than total protein detection. Researchers may need to distinguish wild-type p53 from accumulated mutant p53, or measure post-translational modifications such as phosphorylation, acetylation and methylation to understand p53 activation state and regulatory function.
2. What are human p53 antibodies used for?
A validated human p53 antibody or TP53 antibody supports cancer biology, molecular pathology and translational research workflows. Key research applications include:
- Total p53 protein detection in human cells and tumour tissues
- Wild-type p53 analysis in functional pathway studies
- Mutant p53 accumulation assessment in cancer research models
- DNA damage response and stress signalling studies
- Post-translational modification analysis, including phosphorylation, acetylation and methylation
- Drug response, apoptosis, senescence and tumour biomarker research
- ChIP-based analysis of p53 transcriptional regulation and DNA binding
3. Recommended p53 antibody human products by application
Biorbyt offers a comprehensive portfolio of p53 antibody human products for Western blot, immunohistochemistry, immunofluorescence, flow cytometry, immunoprecipitation and ChIP. The table below highlights total p53, wild-type p53 and modification-specific options for human cancer research.
SKU | Product Name | Applications | Size | Price | Best-Fit Use |
|---|---|---|---|---|---|
P53 Rabbit Polyclonal Antibody (FL-393) – Total p53 Detection | FC, IF, IHC-Fr, IHC-P, WB | 50 μl | $250 | Broad total p53 detection in human cells and tumour samples | |
ChIP, IHC, WB | 100 μl | $600 | DNA-binding, transcriptional regulation and chromatin workflows | ||
ICC, WB | 50 μl | $250 | Wild-type p53 detection and functional pathway analysis | ||
IF, IHC-P, WB | 50 μl | $170 | DNA damage response and phosphorylation-state analysis | ||
FC, ICC, IF, IHC-Fr, IHC-P, WB | 50 μl | $250 | p53 activation, stress signalling and pathway response studies | ||
FC, IF, IHC-Fr, IHC-P | 50 μl | $250 | Transcriptional activation and acetylation-dependent regulation | ||
IF, IHC-Fr, IHC-P, WB | 50 μl | $250 | Methylation-specific p53 regulation and pathway fine-tuning |
4. p53 mutation vs wild-type detection guide
Understanding the difference between wild-type p53 and mutant p53 is essential when selecting a p53 antibody human workflow. These forms can differ in abundance, stability, localization and biological effect in human cancer models.
1
Wild-Type p53
Detect functional stress-responsive p53
Wild-type p53 is normally kept at low basal levels and becomes stabilized after DNA damage or cellular stress. Use high-sensitivity antibodies for WB, combine with phospho-specific antibodies to assess activation, and select wild-type-specific reagents when the biological question requires non-mutant p53 detection.
Recommended products: orb2664 for total detection and orb13631 for wild-type p53 analysis.
2
Mutant p53
Detect accumulated p53 in tumour samples
Mutant p53 proteins are common in human cancers and often show increased stability and accumulation. Total p53 antibodies are frequently suitable for IHC-based tumour studies, biomarker development and cancer model analysis.
Recommended products: orb2664 for strong total p53 signal and orb1930141 for phospho-specific pathway studies.
Best Practice: Total p53 signal alone does not always distinguish wild-type from mutant p53. For human cancer studies, interpret p53 antibody staining alongside TP53 mutation status, protein accumulation patterns, phosphorylation status and downstream pathway readouts where possible.
5. Modification-specific p53 antibodies for activation-state research
Post-translational modifications are critical for understanding p53 activity, stability, localization and transcriptional function. Modification-specific p53 antibodies help researchers move beyond total expression and measure the regulatory state of p53 in response to DNA damage, oncogenic stress or therapeutic treatment.
p53 Modification | Research Meaning | Recommended Product |
|---|---|---|
Phospho-p53 Ser33 | DNA damage response, stress signalling and p53 activation studies | |
Phospho-p53 S315 | Cell cycle, signalling pathway activation and treatment-response analysis | |
Acetyl-p53 K382 | Transcriptional activation, promoter regulation and p53 functional activation | |
Mono-methyl p53 Lys370 | Fine regulation of p53 activity, chromatin-related signalling and pathway control |
Typical Workflow: Treat human cancer cells with DNA-damaging agents or pathway modulators ➔ detect total p53 expression ➔ measure modification-specific p53 signal ➔ evaluate downstream markers of cell cycle arrest, apoptosis, senescence or transcriptional activation.
6. Applications & validation for human p53 antibody workflows
The best human p53 antibody depends on the detection method, sample type and biological question. For tumour tissue staining, IHC performance and nuclear specificity are often the priority. For pathway activation studies, WB and modification-specific antibodies may be more informative. For chromatin biology, ChIP-validated TP53 antibodies are required.
- Western Blot (WB): Detects total p53, wild-type p53 or PTM-specific p53 changes in human cell lysates and cancer models.
- Immunohistochemistry (IHC-P / IHC-Fr): Supports assessment of p53 expression and accumulation patterns in human tumour tissue sections.
- Immunofluorescence / ICC: Enables subcellular localization, nuclear staining and co-staining with DNA damage or apoptosis markers.
- Flow Cytometry (FC): Supports intracellular p53 detection across defined cell populations.
- Immunoprecipitation (IP): Useful for studying p53 interaction partners and regulatory complexes where validated.
- ChIP: Enables analysis of p53 DNA binding and transcriptional regulation using ChIP-validated TP53 antibodies.
7. How to choose the right p53 antibody for human research
Selection should be based on application, sample type, p53 form, modification status and whether the study focuses on expression, localization, mutation-associated accumulation or transcriptional function.
Research Goal | Recommended Antibody Type | Best-Fit Product Examples |
|---|---|---|
Broad p53 detection in human samples | Total p53 antibody | |
Wild-type p53 analysis | Wild-type-specific p53 antibody | |
DNA damage response | Phospho-specific p53 antibody | |
Transcriptional activation | Acetyl-p53 antibody or ChIP-validated TP53 antibody | |
Tumour IHC and biomarker studies | Total p53 antibody with IHC validation |
Selection Tip: For most human cancer studies, begin with a total p53 antibody to assess overall expression, then add wild-type-specific or modification-specific antibodies to answer pathway-specific questions.
8. Companion Bioreagents & Workflow Tools
p53 pathway research often requires multiple reagents to connect p53 expression with DNA damage response, apoptosis, chromatin binding and transcriptional regulation. Biorbyt offers companion products to support complete human p53 research workflows.
Total p53 P53 Rabbit Polyclonal Antibody Broad total p53 detection for WB, IHC, IF, ICC and FC in human cancer research workflows. ChIP Workflow TP53 ChIP Validated Antibody Supports p53 DNA-binding and transcriptional regulation studies in chromatin-based workflows. Pathway Markers DNA Damage Response Antibodies Companion reagents for assessing stress signalling, checkpoint activation and genomic instability pathways. Cell Fate Markers Apoptosis Antibodies Supporting antibodies for measuring downstream p53-dependent apoptosis and treatment response.
9. Scientific Bibliography & Validation Sources
- Levine, A. J. (1997). p53, the cellular gatekeeper for growth and division. Cell, 88(3), 323–331. doi:10.1016/S0092-8674(00)81871-1
- Vousden, K. H., & Prives, C. (2009). Blinded by the light: the growing complexity of p53. Cell, 137(3), 413–431. doi:10.1016/j.cell.2009.04.037
- Kastenhuber, E. R., & Lowe, S. W. (2017). Putting p53 in context. Cell, 170(6), 1062–1078. doi:10.1016/j.cell.2017.08.028
- Bieging, K. T., Mello, S. S., & Attardi, L. D. (2014). Unravelling mechanisms of p53-mediated tumour suppression. Nature Reviews Cancer, 14, 359–370. doi:10.1038/nrc3711
- Olivier, M., Hollstein, M., & Hainaut, P. (2010). TP53 mutations in human cancers: origins, consequences, and clinical use. Cold Spring Harbor Perspectives in Biology, 2(1), a001008. doi:10.1101/cshperspect.a001008