TTNPB

SKU: orb1309111

Description

TTNPB

Research Area

Stem Cell & Developmental Biology

Key Properties

• CAS Number: 71441-28-6
• MW: 348.5
• Purity: >98%
• Formula: C₂₄H₂₈O₂
• SMILES: CC(=Cc1ccc(cc1)C(O)=O)c1ccc2c(c1)C(C)(C)CCC2(C)C
• Target: Retinoid receptor and related
• Solubility: Soluble in DMSO (up to 4 mg/ml).

Bioactivity

• Target IC50:
  RARβ:4.5 nM|RARγ:9.3 nM|RARα:5.1 nM
• In Vivo:
  After 72-hour cultivation in a conditioned medium, TTNPB increases the transcriptional activities of mouse mRARα (EC50: 2.0 nM), β (EC50: 1.1 nM), and γ (EC50: 0.8 nM) in JEG-3 cells. TTNPB exhibits high binding affinity to retinoic acid receptors, thereby inhibiting the binding of [3H]tRA to mRARα (IC50: 3.8 nM), β (IC50: 4.0 nM), and γ (IC50: 4.5 nM). TTNPB inhibits the growth of estrogen receptor-positive breast cancer cells and normal human mammary epithelial cells by inducing a G1 cell cycle arrest. Additionally, TTNPB concentration-dependently reduces the differentiation of ES-D3 cells.
• In Vitro:
  By inducing apoptosis, TTNPB (0.25 mg/kg) can inhibit the growth of the MXT-HI and MXT-HS models.
• Cell Research:
  Human mammary epithelial cells are maintained in Mammary Epithelial Basal Medium (MEBM) supplemented with the Mammary Epithelial Growth Media (MEGM) bullet kit. 184 and 184B5 cells are maintained in MEBM sodium-bicarbonate free (MEBM-SBF) supplemented with the MEGM bullet kit, isoproterenol (10 μM), and transferrin (5 μg/ml). MCF10A cell lines are maintained in DME/F12 containing 5% heat inactivated horse serum, penicillin/streptomycin (100 μg/ml and 100 μg/ml), hydrocortisone (1.4 μM), insulin (10 μg/ml), choleratoxin (100 ng/ml), and EGF (20 ng/ml). Breast cancer cell lines are maintained in Improved MEM Zinc Option containing 10% fetal bovine serum, 1% glutamine, and 1% penicillin/streptomycin. For growth assays, cells are treated with the different retinoids for the specified number of days with media and treatment changes every other day in T47D cells and every 2 days in 184 cells. Cell proliferation is measured according to the protocol for the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay. This colorimetric assay determines the number of viable cells in a sample. Each point represents samples done in quadruplicate.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ro 13-7410; AGN-191183; Arotinoid acid

Compound Identifiers

PubChem CID

5602