TRPS1 antibody

SKU: orb2276224

Description

TRPS1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Breast: A strong TRPS1 staining should be seen in luminal breast epithelial cells. (For low-level TRPS1 detection: Testis: A weak nuclear TRPS1 staining should be seen in spermatogonia.) Negative Control: Colon: Nuclear TRPS1 staining should be absent in epithelial cells. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-512R
• Immunogen: Recombinant fragment (around aa900-1100) of human TRPS1 protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

GC79; LGCR; Transcriptional repressor GATA binding 1; Tricho-rhino-phalangeal syndrome type I protein; Trichorhinophalangeal syndrome I; Trichorhinophalangeal syndrome I homolog; TRPS 1; trpS1; TRPS1 gene; TRPS1_HUMAN; Zinc finger protein GC79; Zinc finger transcription factor TRPS 1 antibody

Appendical epithelial cells are TRPS1 negative but nuclear TRPS1 positivity occurs in stromal cells (Dilution: 1-20).

Intense nuclear TRPS1 staining of luminal (but not basal) breast epithelial cells (Dilution: 1-20).

Invasive breast cancer of no special type (NST) with intense nuclear TRPS1 positivity (Dilution: 1-600).

Quick Database Links

UniProt

Q9UHF7