TRPS1 antibody

• Catalog Number: orb2276225
• Category: Antibodies
• Description: TRPS1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant fragment (around aa900-1100) of human TRPS1 protein (exact sequence is proprietary)
• UniProt ID: Q9UHF7
• Tested applications: IHC
• Dilution range: 1:100-1:200
• Application notes: Positive Control: Breast: A strong TRPS1 staining should be seen in luminal breast epithelial cells. (For low-level TRPS1 detection: Testis: A weak nuclear TRPS1 staining should be seen in spermatogonia.) Negative Control: Colon: Nuclear TRPS1 staining should be absent in epithelial cells. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Clone Number: Biorbyt-512R
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GC79; LGCR; Transcriptional repressor GATA binding
• Note: For research use only

Appendical epithelial cells are TRPS1 negative but nuclear TRPS1 positivity occurs in stromal cells (Dilution: 1-20).

Intense nuclear TRPS1 staining of luminal (but not basal) breast epithelial cells (Dilution: 1-20).

Invasive breast cancer of no special type (NST) with intense nuclear TRPS1 positivity (Dilution: 1-600).