Trovafloxacin

SKU: orb3029199

Description

Trovafloxacin, a broad-spectrum quinolone antibiotic, exhibits potent activity against Gram-positive bacteria.

Research Area

Infectious Disease & Virology, Molecular Biology

Key Properties

• CAS Number: 147059-72-1
• MW: 416.35
• Purity: 99.21%
• Formula: C₂₀H₁₅F₃N₄O₃
• SMILES: O=C(C1=CN(C2=CC=C(F)C=C2F)C3=NC(N4C[C@@]5([H])[C@H](N)[C@@]5([H])C4)=C(F)C=C3C1=O)O
• Target: Antibiotic,Topoisomerase,Antibacterial,DNA gyrase
• Solubility: DMSO:4.17 mg/mL (10.02 mM);H2O:Insoluble

Bioactivity

• Target IC50:
  PANX1:4 μM (IC50)
• In Vivo:
  In male C57BL/6 J mice, TNF-induced p65 nuclear translocation disrupted by Trovafloxacin (150 mg/kg; oral administration). Trovafloxacin treatment increases expression of early NF-κB-related factors A20 and IκBα. Trovafloxacin increased serum levels of alanine amino transferases (ALT) and pro-inflammatory cytokines when administered in combination with lipopolysaccharide (LPS) or TNF to mice induces severe liver toxicity associated with vast apoptotic areas in the liver.
• In Vitro:
  In HepG2 cells, Trovafloxacin (20 μM; 24 hours; ) and tumor necrosis factor (TNF; 4 ng/mL) incubation induces apoptosis and increases leakage of lactate dehydrogenase (LDH). Trovafloxacin (20 μM; 24 hours; HepG2 cells) and TNF (4 ng/mL) incubation increases expression of early NF-κB-related factors A20 and IκBα. Trovafloxacin prolongs TNF-induced activation of MAPKs and IKKα/β activation in HepG2. Trovafloxacin is a potent TO-PRO-3 uptake inhibitor by apoptotic cells. Trovafloxacin also inhibits ATP release from apoptotic cells. Trovafloxacin is equally active against both penicillin-susceptible and -resistant pneumococci, with MICs of 0.06-0.25 mg/mL reported for more than 700 isolates. The MICs of Trovafloxacin at which 90% of isolates are inhibited for 55 isolates of pneumococci is 0.125 μg/mL.

Storage & Handling

• Storage: store under nitrogen,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA gyrase, DNA, DNAgyrase, broad-spectrum, Antibiotic, Bacterial, bacterial, Inhibitor, hepatotoxicity, gyrase, inhibit, Pannexin 1 channel (PANX1), LPS, NF-κB, pneumococci, PANX1, pro-inflammatory, Topoisomerase, topoisomerase-IV, TNF, Trovafloxacin

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Gene Symbol

Antibiotic,Topoisomerase,Antibacterial,DNA gyrase