Troglitazone

SKU: orb1304465

Description

Troglitazone

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 97322-87-7
• MW: 441.54
• Purity: 98.48%
• Formula: C₂₄H₂₇NO₅S
• SMILES: Cc1c(C)c2OC(C)(COc3ccc(CC4SC(=O)NC4=O)cc3)CCc2c(C)c1O
• Target: Autophagy,PPAR,Ferroptosis,Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.53 mM);Ethanol:4.4 mg/mL (9.97 mM);DMSO:18.33 mg/mL (41.51 mM)

Bioactivity

• Target IC50:
  PPARγ (murine):780 nM (EC50)|PPARγ (human):550 nM (EC50)
• In Vivo:
  Local hypoxia is observed in liver but not in adipose tissue in troglitazone-treated mice. A significant increase in Ki-67/CD31 LI in liver, BAT, and WAT is observed in the 400 mg/kg troglitazone group. Troglitazone reduce the incidence of diabetes by 16 weeks compared to controls, when administered by gavage from weaning at a dose of 400 mg/kg body weight.
• In Vitro:
  Troglitazone inhibit the growth of 786-O and A498 cells with EC50 Values of 5.71 μM and 8.38 μM. Troglitazone(5 μM) significantly inhibit the CYP2C8-dependent paclitaxel 6a-hydroxylation and CYP2C9-dependent S-warfarin 7-hydroxylation. Troglitazone extensively suppresses CYP2C19-dependent S-mephenytoin 4′-hydroxylation and CYP3A4-dependent testosterone 6b-hydroxylation and moderately influence the CYP1A2- and CYP2B6-dependent 7-ethoxycoumarin O-deethylation, CYP2A6-dependent coumarin 7-hydroxylation and CYP2D6-dependent bufuralol 1′-hydroxylation. CYP2E1-dependent 7-ethoxycoumarin O-deethylation is not inhibited.
• Cell Research:
  Troglitazone is made at 100 mM concentration in DMSO and added to the culture medium a the final concentration of less than 0.1% the effect of PPARγ li and on cell proliferation of RCC cells is determined using MTT assay. Briefly, cells of 0. × 04 cells/well are inoculated into a 96-well plate, treated with pioglitazone or troglitazone at various concentration . After an incubation for 24 h, 20 μL well 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, 5 g/L) is added to each well the medium is then removed, and 200 μL of 0.04mol/LHCl in isopropanol is added to dissolve the reduced formazan product the plate is read in a microplate reader at 590 nM.

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

CS045, CS-045, CS 045, Apoptosis, Autophagy, Ferroptosis, inhibit, Noscal, Inhibitor, PPAR, Peroxisome proliferator-activated receptors, PPARγ, Prelay, Rezulin, Romglizone, Romozin, Troglitazone

Compound Identifiers

PubChem CID

5591