TRITC IF Detection System Kit (Rabbit Anti-Sheep) [Out of stock]

• Catalog Number: orb219884
• Category: Tools
• Description: TRITC IF Detection System Kit (Rabbit Anti-Sheep)
• Tested applications: ICC, IF
• Storage: Store at 4°C for one year.
• Note: For research use only
• Application notes: Super Sensitive TRITC IF Detection System Kit is a non-biotin, one-step detection system that allows for the demonstration of antigens in paraffin-embedded tissue, cryostat sections, and cell preparations. This kit has been developed using a proprietary hyper labeling technology used to label IgG directly with more Tetramethylrhodamine (TRITC). One step polymer system provides increased sensitivity, time savings and detection simplicity. All the components contain with PBS, proteins, stabilizers and preservatives. This kit is suitable for single, double and triple immunofluorescence detect. The color is emerald green with a correct result. The TRITC is easily faded with light; all experiment process need keep away from light.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber, and detected using Super Sensitive TRITC IF Detection System Kit.