Triptohypol C

SKU: orb3054219

Description

Dihydrocelastrol is synthesized by hydrogenation of celastrol, a treterpene isolated from Chinese medicinal plant Tripterygium regelii. Dihydrocelastrol could inhibit cell proliferation and promote apoptosis through caspase-dependent way in vitro.

Key Properties

• CAS Number: 193957-88-9
• MW: 452.63
• Purity: >98% (HPLC)
• Formula: C₂₉H₄₀O₄
• Solubility: DMSO : 16.67 mg/mL (36.83 mM; ultraphonic; )

Bioactivity

• In Vivo:
  Caused 3.12% apoptosis in cells, which was less cytotoxic than Tripterin (>10%). Animal model: Zebrafish. Dosage: 0.5 μM, 1 μM and 1.25 μM. Administration: 72 h. Result: Had less effect than Tripterin on the death rate and malformation of zebrafish either at a concentration of 1.25 μM for 24 h or at a concentration of 0.5 μM for 72 h.
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• In Vitro:
  Triptohypol C (compound 3a) (2 μM; 1 h) strongly antagonize the effect of TNFα on inducing IκBα degradation, and inhibits inflammatory response by promoting the interactions of Nur77 with TRAF2 and p62/SQSTM1.Triptohypol C (2 μM; 10 h) cause 3.12% apoptosis in HepG2 cells, which is less toxic than Tripterin. Western blot analysis. Cell line: Lysates from HepG2 cells (incubated with 20 ng/mL TNFα for 30 min). Concentration: 2 μM. Incubation time: 1 h. Result: Strongly antagonized the effect of TNFα on inducing IκBα degradation Immunofluorescence Cell line: HepG2 cells (transfected with Myc-Nur77 and Flag-TRAF2 or Flag-p62). Concentration: 2 μM. Incubation time: 1 h. Result: Promoted the interactions between Nur77 and TRAF2 and p62/SQSTM1.Apoptosis Analysis Cell line: HepG2 cells. Concentration: 2 μM. Incubation time: 10 h. Result: Caused 3.12% apoptosis in cells, which was less cytotoxic than Tripterin (> 10%).
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only