TRIM29 antibody

SKU: orb2276285

Description

TRIM29 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: A strong membranous and cytoplasmic TRIM29 immunostaining should be seen in squamous epithelial cells. Negative Control: Tonsil: All non-epithelial cells must not show any TRIM29 immunostaining. Cellular Localization: Cytoplasmic and cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: Biorbyt-629M
• Immunogen: Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Ataxia telangiectasia group D complementing gene (ATDC); Tripartite motif-containing protein 29 (TRIM29)

Anal canal Squamous cell carcinoma with strong TRIM29 immunostaining of tumor cells.

Prostate A moderate intensity predominantly membranous TRIM29 staining is seen in basal cells of the prostate.

Tonsil surface epithelium A strong membranous and cytoplasmic TRIM29 immunostaining is seen in squamous epithelial cells of the tonsil surface.

Quick Database Links

UniProt

Q14134