TPK1 Antibody

SKU: orb41306

Description

This TPK1 Antibody is an unconjugated polyclonal product. It tagets TPK1 using a Recombinant Human Thiamin pyrophosphokinase 1 protein (1-243AA) as the immunogen. This antibody is suitable for ELISA, IF, IHC, IP, WB. Purification: Antigen Affinity Purified.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 1:500-1:2000 , IHC:1:20-1:200
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant Human Thiamin pyrophosphokinase 1 protein (1-243AA)
• Target: TPK1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

hTPK1 antibody; Placental protein 20 antibody; PP20 antibody; Thiamin pyrophosphokinase 1 antibody; Thiamine diphosphokinase antibody; Thiamine kinase antibody; Thiamine pyrophosphokinase 1 antibody; THMD5 antibody; TPK1 antibody; TPK1_HUMAN antibody

Positive WB detected in: U251 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), K562 whole cell lysate(30µg), CT-26 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), JK whole cell lysate(30µg), Mouse Liver tissue lysate(30µg), Mouse Kidneyr tissue lysate(30µg). All lanes: TPK1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/40000 dilution. Predicted band size: 27,14kDa, Observed band size: 27 kDa, Exposure time: 180s.

IHC image of orb41306 diluted at 1:100 and staining in paraffin-embedded human Kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

IHC image of orb41306 diluted at 1:100 and staining in paraffin-embedded human Heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

IHC image of orb41306 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

Quick Database Links

Gene Symbol

TPK1

UniProt

Q9H3S4