Human Tau-Protein Kinase 1 (tPK1) ELISA Kit

SKU: orb776594

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tau-Protein Kinase 1(tPK1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human tPK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human tPK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human tPK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human tPK1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TTBK1 ELISA Kit, BDTK ELISA Kit, TTK ELISA Kit, Cdk5/P20 ELISA Kit, CDK5/P23 ELISA Kit, GSK ELISA Kit, STK31 ELISA Kit, Brain-derived tau kinase ELISA Kit, Tau-Tubulin Kinase 1 ELISA Kit, Tau-Protein O-Hosphotransferase ELISA Kit, Brain Protein Kinase PK40erk ELISA Kit