TPE-MI

SKU: orb1983093

Description

TPE-MI is an aggregation-induced emission (AIE) luminogen that selectively labels live cells and covalently traps cysteine residues in unfolded proteins. It is a valuable tool for studying proteome damage, such as in malaria parasite research following dihydroartemisinin treatment, in both in vitro and in vivo experimental models.

Research Area

Infectious Disease & Virology, Neuroscience

Key Properties

• CAS Number: 1245606-71-6
• MW: 441.52
• Purity: 97.17%
• Formula: C₃₁H₂₃NO₂
• SMILES: O=C1C=CC(=O)N1C2=CC=C(C=C2)C(C=3C=CC=CC3)=C(C=4C=CC=CC4)C5=CC=C(C=C5)C
• Target: Huntingtin,Parasite,Others
• Solubility: DMSO:40 mg/mL (90.6 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:2 mg/mL (4.53 mM)

Bioactivity

• Cell Research:
  TPE-MI cell staining 1. Solution preparation: TPE-MI is dissolved in DMSO as a 1 or 2 mM stock solution. The stock solution is stored at 4°C in the dark. 2. Operation steps 1. Cultivate cells to a suitable state, rinse with PBS to remove residual culture medium, and treat with freshly diluted TPE-MI (50 μM PBS solution) at 37°C for 30 minutes; 2. Rinse with PBS to remove residual TPE-MI solution; 3. Application examples 1) Flow cytometry analysis: Resuspend cells in PBS, precipitate by centrifugation (120 g reaction, 6 minutes), and then resuspend in 250 μL PBS in a flow cytometry tube for detection and analysis. 2) Fluorescence imaging: Fix cells on the plate with 4% paraformaldehyde. 3) SDS-PAGE: The stained cells were lysed on the plate by adding native lysis buffer and analyzed by 12% acrylamide SDS-PAGE.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tetraphenylethene maleimide

Compound Identifiers

PubChem CID

68227137