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Description
Research Area
Cancer Biology
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to inhibit TNF-beta-mediated cytotoxicity using L929 Mouse fibrosarcoma cells. The ED50 for this effect is typically 2. 5-10μg/ml in the presence of 1 ng/mL of recombinant human TNF-beta. |
| Target | TNFRSF14 |
| Tag | C-Fc Tag |
| MW | 45.4 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Leu 39 - Val 202 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFRSF14, ATAR, HVEA, HVEM, LIGHTR, TR2, CD270
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003811.2 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
Available Sizes
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